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Table 1 VCM concentrations (mean ± SD) and minimum VCM bioactivities

From: Double-layered antibiotic-loaded cement spacer as a novel alternative for managing periprosthetic joint infection: an in vitro study

  Day 1 3 7 14 28 56 84
D-L spacer
(μg/mL)
VCM concentration
measured by HPLC
1415.3
(± 346.9)
175.6
(± 76.2)
152.8
(± 45.2)
109.3
(± 29.3)
59.5
(± 16.1)
29.4
(± 10.8)
22.6
(± 7.3)
Minimum
VCM bioactivity
> 320 160 160 80 40 20 20
PMMA spacer
(μg/mL)
VCM concentration
measured by HPLC
1580.1
(± 1312.9)
179.5
(± 81.1)
66.1
(± 45.5)
19.4
(± 10.3)
8.5
(± 4.6)
3.1
(± 1.2)
1.9
(± 0.7)
Minimum
VCM bioactivity
> 320 160 40 10 2.5 0 0
  1. Bioactivity was converted to concentration (μg/mL) by multiplying the MIC by the smallest eluent dilution rate that prevented the inhibition of MRSA growth. The values obtained by high-performance liquid chromatography (HPLC) and the broth microdilution method showed very similar values and patterns