Fig. 1

Experimental workflow of the study. Bone morphometry and a mechanical test were performed on the same sample. First, X-ray micro-computed tomography was undergone and three-dimensional reconstruction of the mid-diaphysis and distal metaphysis regions of interest was done. Trabecular bone and cortical bone were separately analyzed at the distal metaphysis. After reconstruction, morphological, tissue mineral density (TMD), and cortical porosity analyses were calculated. After acquiring images, samples were subjected to a three-point mechanical test