Skip to main content

Table 3 Population doubling level of disc cells cultured in different culture media

From: Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells

PD

alpha-MEM

DMEM

Ham’s F-12

alpha/F-12

DMEM/alpha

DMEM/F-12

AF

P0

3.5 ± 0.1

3.3 ± 0.4

2.9 ± 0.1

3.3 ± 0.1

3.5 ± 0.3

3.4 ± 0.2

P1

3.3 ± 0.4

3.1 ± 0.8

2.6 ± 0.6

2.6 ± 0.4

3.0 ± 0.5

2.8 ± 0.6

P2

3.3 ± 0.5

3.1 ± 0.0

3.1 ± 0.6

3.1 ± 0.6

3.4 ± 0.3

3.1 ± 0.6

 

cPDL

10.2 ± 0.2 *#

9.5 ± 0.4

8.5 ± 0.8

9.0 ± 0.7

9.9 ± 0.7 *

9.3 ± 0.7

NP

P0

3.7 ± 0.4

3.3 ± 0.5

3.1 ± 0.4

2.9 ± 0.2

2.7 ± 0.7

2.8 ± 0.5

P1

3.0 ± 0.1

2.8 ± 0.3

2.5 ± 0.3

2.5 ± 0.4

2.7 ± 0.2

2.8 ± 0.0

P2

2.9 ± 0.2

2.9 ± 0.2

2.1 ± 0.3

2.5 ± 0.2

2.8 ± 0.3

2.8 ± 0.3

 

cPDL

9.6 ± 0.3*#+§

9.1 ± 0.6*#

7.7 ± 0.5

7.8 ± 0.6

8.2 ± 0.2

8.3 ± 0.5

  1. PD Population doubling in each passage P0, P1 and P2; cPDL cumulated PD level at the end of passage P2; AF annulus fibrosus; NP nucleus pulposus. Data is shown as mean ± SD (AF n = 5, NP n = 3).
  2. *Significant (p value < 0.05) compared to Ham’s F-12
  3. #Significant (p value < 0.05) compared to alpha/F-12
  4. +Significant (p value < 0.05) compared to DMEM/alpha
  5. §Significant (p value < 0.05) compared to DMEM/F-12