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Table 2 Primer and probes

From: Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells

Gene title

Accession number#

Primer sequence

UPL

 

forward

reverse

probe

ACAN

NM_001135.3

NM_013227.3

ctggaagtcgtggtgaaagg

tcgagggtgtagcgtgtaga

21

COL1A1

NM_000088.3

gggattccctggacctaaag

ggaacacctcgctctcca

67

COL2A1

NM_001844.4

NM_033150.2

ccctggtcttggtggaaa

cattggtccttgcattactcc

19

FOXF1

NM_001451.2

cagcctctccacgcactc

cctttcggtcacacatgct

5

KRT18

NM_000224.2

NM_199187.1

aagctggaggctgagatcg

tccaaggcatcaccaagatta

70

ATP5FB1*

NM_001686.3

agaggtcccatcaaaaccaa

tcctgctcaacactcatttcc

50

RPL13A*

NM_012423.3

NM_00127049.1

caagcggatgaacaccaac

tgtggggcagcatacctc

28

  1. *Housekeeping genes used as reference genes in normalisation for calculation of mRNA expression level
  2. #Includes all transcript variants of this gene covered by the chosen primers