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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells

Fig. 3

Glycosaminoglycan production through cell culture of annulus fibrosus and nucleus pulposus cells in different media. a Glycosaminoglycan (GAG) deposition in cells of passage P0, P1 and P2 before reaching 100% confluency (exemplary for female, 52 years, cells cultured in alpha-MEM) visualised by alcian blue staining (GAGs in blue, cell nuclei in pink). Scale bar 100 μm. b Total amount of secreted GAGs by cells within passage P0, P1 and P2 (shown as mean ± SD, AF n = 5, NP n = 3). *Significance (p value < 0.05) within same passage

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X