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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Egr-1 increases angiogenesis in cartilage via binding Netrin-1 receptor DCC promoter

Fig. 2

DCC regulates the angiogenesis in OA via VEGF. a Netrin-1 expression in OA cartilage. Results are expressed as the ratio of Netrin-1 and GAPDH (*P = 0.00009 vs. the sham, n = 5 in each group). b DCC expression in OA cartilage. Results are expressed as the ratio of DCC and GAPDH (*P = 0.0012 vs. the sham, n = 5 in each group). c VEGF expression in DCC siRNA-treated OA cartilage. Results are expressed as the ratio of VEGF and GAPDH (*P = 0.0002 vs. the others, #P = 0.0003 vs. OA group and scramble siRNA-treated group, n = 5 in each group). d Co-immunoprecipitation of DCC and VEGF in OA cartilage. The lysates were immunoprecipitated with DCC antibody and immunoblotted with VEGF antibodies (*P = 0.0008 vs. sham, n = 4, lane 1 = positive control, lane 2 = negative control, lane 3 = sham, lane 4 = OA group. For the positive control, VEGF antibody was used, and for the negative control, IgG was used instead of DCC antibody as the immunoprecipitants)

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