Fig. 7

Effect of alcohol on in vitro MSC migration. Bar graphs show a migration of primary MSCs as a function of rOPN concentration and alcohol treatment and b chemotactic index of MSC migration data. Control = MSC migration in the absence of rOPN, no EtOH = rOPN stimulated MSC migration with no exposure to alcohol, pre-exposure = 24 h. pre-incubation of MSC in 50 mM EtOH, concurrent exposure = MSC migration assay performed in the presence of 50 mM EtOH in the rOPN well, pre- and concurrent exposure = combination of pre-exposure and concurrent exposure treatments. Data are shown as mean ± SEM, n = 4 experiments/group, each performed in triplicate. A two-way ANOVA with Holm-Sidak post hoc testing was performed on pre-defined comparisons of the no EtOH group versus every other bar at either the 5 or 1 μg/mL rOPN concentration and comparing no EtOH at the 5 and 1 μg/mL rOPN concentrations. *p < 0.05 comparing no EtOH at the 5 and 1 μg/mL rOPN concentrations. +p < 0.05 comparing no EtOH to all other bars in the 5 μg/mL rOPN group (to left of dashed line). All conditions showed statistically less MSC migration than the no EtOH group. ^p < 0.05 comparing no EtOH to all other bars in the 1 μg/mL rOPN group (to right of dashed line). All conditions except pre-exposure showed statistically less MSC migration than the no EtOH group. Each experiment was repeated at least three times utilizing unique primary MSC cultures