Fig. 5

Osteopontin-1 protein levels in fracture callus at a 3 and b 7 days post-fracture. Bar graphs show fracture callus OPN levels. Below the graphs are representative western blots for the respective treatment groups and time points. For western blots, CS = contralateral tibia saline control group, CA = contralateral tibia alcohol-exposed group, FS = fracture callus from saline control group, and FA = fracture callus from alcohol-exposed group. Bar graph are shown as mean ± SEM, n = 3–4/group for contralateral, and 8–9/group for fracture callus. *p < 0.05 by one-way ANOVA with Tukey’s post hoc test