Fig. 4

Micro-CT analysis of fracture callus at day 7 post-fracture. Representative 3D reconstructions of a saline control and b alcohol-exposed fracture calluses. The white area is mature, pre-existing bone; black area is mineralized tissue formed since fracture; gray area is soft tissue (based on segmentation as described in the “Methods” section). There is more newly mineralized tissue seen in the saline control callus than in callus from alcohol-treated mice. c Quantification of soft tissue, total newly mineralized tissue, and newly mineralized tissue in the external callus as a percent of the total callus volume (%TV). There is significantly less newly mineralized tissue in the external callus from alcohol-exposed mice compared to saline controls. Data are shown as mean ± SEM, n = 5/group. *p = 0.03 by Student’s t test