Fig. 2

The effect of BET protein inhibitor on regulation of matrix degradation enzyme genes transcription in human chondrocytes (a–g). The transcriptional expression (RT-PCR) of MMP1, MMP3, MMP13, ADAMTS4, MMP2, MMP9, and ADAMTS5 genes in human chondrocytes, respectively, after the cells were pre-treated with or without I-BET151 (1 μM) followed by addition of IL-1β (10 ng/ml) or TNF-α (10 ng/ml) for 24 h. Relative fold change values were calculated in comparison with vehicle control set as 1 (n = 3)