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Table 2 Expression levels of osteogenic or chondrogenic genes after cell co-culture (2-ΔCT method)

From: Effect of modified compound calcium phosphate cement on the differentiation and osteogenesis of bone mesenchymal stem cells

Target genes Avg.ΔCt (target gene Ct–GAPDH Ct) ΔΔCt (avg.ΔCt, target-avg.ΔCt control) Target gene amount relative to control 2-ΔΔCt
Collagen I (a) 10.98 + 0.28 0.00 + 0.28 1.0(0.8–1.2)
Collagen I (b) 11.18 + 0.26 0.20 + 0.38 0.9(0.7–1.1)
Collagen I (c) 10.07 + 0.17 −0.91 + 0.33 1.9(1.5–2.3)
Collagen I (d) 8.22 + 0.25 −2.76 + 0.38 6.8(5.2–8.8)
Collagen II (a) 13.15 + 0.20 0.00 + 0.20 1.0(0.9–1.1)
Collagen II (b) 12.89 + 0.17 −0.24 + 0.26 1.2(1.0–1.4)
Collagen II (c) 11.58 + 0.23 −1.57 + 0.30 3.0(2.4–3.7)
Collagen II (d) 10.64 + 0.18 −2.51 + 0.27 5.7(4.7–6.9)
Aggrecan (a) 10.87 + 0.19 0.00 + 0.19 1.0(0.9–1.1)
Aggrecan (b) 10.34 + 0.30 −0.53 + 0.35 1.4(1.2–1.8)
Aggrecan (c) 9.27 + 0.52 −1.60 + 0.55 3.0(2.1–4.3)
Aggrecan (d) 7.62 + 0.30 −3.25 + 0.35 9.5(7.7–11.7)
AKP (a) 11.57 + 0.40 0.00 + 0.40 1.0(0.8–1.3)
AKP (b) 11.70 + 0.52 0.13 + 0.65 0.9(0.6–1.4)
AKP (c) 9.67 + 0.25 −1.90 + 0.47 3.7(2.7–5.1)
AKP (d) 8.27 + 0.28 −3.30 + 0.49 9.8(7.0–13.8)
OSX (a) 14.24 + 0.26 0.00 + 0.26 1.0(0.8–1.2)
OSX (b) 13.86 + 0.21 −0.38 + 0.33 1.3(1.0–1.6)
OSX (c) 12.53 + 0.24 −1.71 + 0.35 3.3(2.6–4.2)
OSX (d) 11.10 + 0.16 −3.14 + 0.31 8.8(7.1–10.2)