Fig. 3

Effects of cytokines and PGE2 on CGRP and CGRP receptor expression in synovial cell culture. Real-time polymerase chain reaction analysis for a calcitonin gene-related peptide (CGRP), b receptor activity-modifying protein 1 (RAMP1), and c calcitonin receptor-like receptor (CLR) gene expression in synovial cell culture. Synovial cells were stimulated with human recombinant IL-6 (100 ng/ml), IL-1β (50 ng/ml), TNF-α (10 ng/ml), or PGE2 (10 μM) for 24 h prior to the extraction and analysis of total RNA. All data are presented as the mean ± standard error (n = 6). *p < 0.05 compared with the untreated control