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Table 1 RT-PCR primer for differentiation-specific gene expression analysis

From: Characterization of progenitor cells derived from torn human rotator cuff tendons by gene expression patterns of chondrogenesis, osteogenesis, and adipogenesis

Primer

Sequences

Annealing temperature

Cycle

ALP

Forward: 5′-CCCAAAGGCTTCTTCTTG-3′

55 °C

35

 

Reverse: 5′-CTGGTAGTTGTTGTGAGC-3′

  

Osteopontin

Forward: 5′-ACGCCGACCAAGGAAAACTC-3′

60 °C

40

 

Reverse: 5′-GTCCATAAACCACACTATCACCTCG-3′

  

PPAR-γ

Forward: 5′-TGGGTGAAACTCTGGGAGATTC-3′

60 °C

35

 

Reverse: 5′-CATGAGGCTTATTGTAGAGCTG-3′

  

LPL

Forward: 5′-GAGATTTCTCTGTATGGCACC-3′

60 °C

30

 

Reverse: 5′-CTGCAAATGAGACACTTTCTC-3′

  

SOX9

Forward: 5′-AACATGACCTATCCAAGCGC-3′

55 °C

35

 

Reverse: 5′-ACGATTCTCCATCATCCTCC-3′

  

COL II

Forward: 5′-TCTGCAACATGCAGACTGGC-3′

57 °C

40

 

Reverse: 5′-GAAGCAGACAGGCCCTATGT-3′

  

COL X

Forward: 5′-GCCCAAGAGGTGCCCCTGGAATAC-3′

57 °C

35

 

Reverse: 5′-CCTGAGAAAGAGGAGTGGACATAC-3′

  

GAPDH

Forward: 5′-CCACCCATGGCAAATTCCATGGCA-3′

55 °C

30

 

Reverse: 5′-TCTAGACGGCAGGTCAGGTCCACC-3′