Primer | Sequences | Annealing temperature | Cycle |
---|---|---|---|
ALP | Forward: 5′-CCCAAAGGCTTCTTCTTG-3′ | 55 °C | 35 |
Reverse: 5′-CTGGTAGTTGTTGTGAGC-3′ | |||
Osteopontin | Forward: 5′-ACGCCGACCAAGGAAAACTC-3′ | 60 °C | 40 |
Reverse: 5′-GTCCATAAACCACACTATCACCTCG-3′ | |||
PPAR-γ | Forward: 5′-TGGGTGAAACTCTGGGAGATTC-3′ | 60 °C | 35 |
Reverse: 5′-CATGAGGCTTATTGTAGAGCTG-3′ | |||
LPL | Forward: 5′-GAGATTTCTCTGTATGGCACC-3′ | 60 °C | 30 |
Reverse: 5′-CTGCAAATGAGACACTTTCTC-3′ | |||
SOX9 | Forward: 5′-AACATGACCTATCCAAGCGC-3′ | 55 °C | 35 |
Reverse: 5′-ACGATTCTCCATCATCCTCC-3′ | |||
COL II | Forward: 5′-TCTGCAACATGCAGACTGGC-3′ | 57 °C | 40 |
Reverse: 5′-GAAGCAGACAGGCCCTATGT-3′ | |||
COL X | Forward: 5′-GCCCAAGAGGTGCCCCTGGAATAC-3′ | 57 °C | 35 |
Reverse: 5′-CCTGAGAAAGAGGAGTGGACATAC-3′ | |||
GAPDH | Forward: 5′-CCACCCATGGCAAATTCCATGGCA-3′ | 55 °C | 30 |
Reverse: 5′-TCTAGACGGCAGGTCAGGTCCACC-3′ |