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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Transmission of ER stress response by ATF6 promotes endochondral bone growth

Fig. 5

ATF6 stimulates chondrocyte hypertrophy, mineralization, and endochondral bone growth. a, b Safranin O/fast green staining of metatarsal bones. Metatarsals from 15-day-old mouse embryos were cultured in the absence (CTR) or presence of Ad-ATF6 (MOI = 50) for 7 days and stained with safranin O/fast green, shown in low-power (a) and high-power (b) microphotographs. c Quantification of the length of the proliferating (P) zone and hypertrophic (H) zone in growth plates of metatarsal bones. *p < 0.05 versus control by paired Student’s t test. d Alizarin red/Alcian Blue staining of metatarsals. Explants were fixed and processed for staining. A representative photograph of an explanted metatarsal is presented. e Percentage changes in total (T) and mineralization (M) length of metatarsal bones. Percentage changes in bone length were calculated as (length at d5-length at d0)/length at d0. *p < 0.05 versus control; scale bar = 100 μm. Error bars, SD

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