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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Transmission of ER stress response by ATF6 promotes endochondral bone growth

Fig. 4

Expression of ATF6 in the growth plate chondrocytes in vivo. a Immunohistochemistry of ATF6 in the section of long bone from a 15-day-old mouse embryo. Metatarsals from 15-day-old mouse embryos were cultured in the absence (Ad-GFP, serves as a control) or presence of Ad-ATF6 (MOI = 50) for 7 days, then processed for paraffin embedding and sections were performed. The section was stained with anti-ATF6 polyclonal antibody (brown) and counterstained with Mayer’s hematoxylin (blue). Immunostaining for ATF6 demonstrates localization of strongly immunopositive chondrocytes in the lower proliferative/upper hypertrophic zones of the growth plate. The expression of ATF6 dramatically increased after infected with Ad-ATF6, the scale bars represent 100 μm. The red arrow is protein ATF6. b, c Metatarsals from 15-day-old mouse embryos were cultured in the absence (Ad-GFP) or presence of Ad-ATF6 (MOI = 50) for 5 days, and the mRNA levels of Col X (b) and MMP13 (c) were determined using real-time PCR. The normalized values were calibrated against control (CTR). The asterisk indicates significant increase or decrease from control (*p < 0.05)

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