Fig. 1

Expression of ATF6 and ATF6a in the course of chondrogenesis in a micromass culture of ATDC5 cells. a–d Real-time PCR assay. Total RNA was prepared from micromass cultures of ATDC5 cells in the presence of 300 ng/ml recombinant BMP2 for various time points, as indicated, and the mRNA expression of ATF6a, collagen II, collagen X, MMP13, and GAPDH (serving as an internal control) were examined by real-time PCR. e Western blotting assay. After incubation of micromass cultures of ATDC5 cells with 300 ng/ml BMP2 for the times indicated, the cells were lysed, and 40-mg protein samples were assayed for ATF6, ATF6a, collagen X, and tubulin (serving as an internal control) by Western blotting with anti-ATF6, anti-collagen X, or anti-tubulin, respectively. The arrow is protein ATF6, ATF6a, and collagen X, respectively