Fig. 2
From: Nanosecond pulsed electric field inhibits proliferation and induces apoptosis in human osteosarcoma
Apoptosis induction at different times post nsPEF treatment. After 40 kV/cm and 30 pulses of nsPEF treatment, the control and treated cells were stained with Hoechst 33528 (a upper lane) and TUNEL (a lower lane). The statistical analysis of the positive apoptotic cells were counted and shown in (b) at different hours (0, 3, 12, 24, and 48 h). The apoptotic cells were significant in 24 h post nsPEF treatment. The cell cycle was analyzed by flow cytometry (c) and statistically analyzed in (d), which indicates that nsPEF arrest cells in the G0/G1 phase