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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Nanosecond pulsed electric field inhibits proliferation and induces apoptosis in human osteosarcoma

Fig. 1

NsPEF treatment parameter optimizing by CCK-8 and flow cytometry. After 24 h post nsPEF, CCK-8 assay was used to calculate the IC50 values under different electric field strengths (a) and different pulse numbers (d). The flow cytometry was used to detect apoptotic histograph under different electric field strengths (b) and different pulse numbers (e) as well as the apoptotic cell rate under different electric field strengths (c) and different pulse numbers (f). There was significant (P > 0.001) growth inhabitation when electric field strength was 30, 40, and 50 kV/cm (a) and when pulse number was 30 (1D) vs control. The apoptotic cell rate is significantly increased when electric field strength was 40–50 kV/cm (b, c) and when pulse number was 30 (e, f)

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