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Table 1 Reagents

From: Acceleration of bone-defect repair by using A-W MGC loaded with BMP2 and triple point-mutant HIF1α-expressing BMSCs

Name Company
Adv-HIF1αmu-hrGFP (1.6 × 108 pfu/mL) First Affiliated Hospital of Liaoning Medical Tissue Engineering Laboratory
Adv-BMP2-hrGFP (2.0 × 108 pfu/mL) First Affiliated Hospital of Liaoning Medical Tissue Engineering Laboratory
Rabbit anti-human HIF1α monoclonal antibody, rabbit anti-human BMP2 monoclonal antibody  
Secondary antibodies, β-actin Santa Cruz, USA
DMEM (low glucose, catalog number: 10567-014), trypsin, fetal bovine serum Gibco, USA
A-W MGC biological scaffolds First Affiliated Hospital of Liaoning Medical Tissue Engineering Laboratory
ALP kit TaKaRa, Japan
Other reagents First Affiliated Hospital of Liaoning Medical Tissue Engineering Laboratory
  1. HIF1αmu hypoxia-inducible factor 1 mutation, hrGFP human renilla reniformis green fluorescent protein, BMP2 bone morphogenetic protein 2, ALP alkaline phosphatase, A-W MGC apatite-wollastonite magnetic bioactive glass-ceramic
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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X