3D micro-CT analysis of femurs after grafting of demineralized allogenic bone powder loaded with bFGF-PKD-CBD. 3D micro-CT images of fractured mouse femurs treated with (A) PBS/DBP, (B) 0.58 nmol bFGF/DBP, (C) 0.058 nmol bFGF-PKD-CBD/DBP, and (D) 0.58 nmol bFGF-PKD-CBD/DBP at 6 weeks. Green: newly formed bone; gray: existing bone. The scale bars indicate 3 mm. (E) Callus area and (F) bone mineral content at fracture sites for the indicated treatment groups. Data are presented as the mean ± standard error (S.E.; error bars; n = 6). a: P < 0.05 compared with the control group (PBS/DBP). b: P < 0.05 compared with the 0.58 nmol bFGF group.