Figure 2

Quantification of transduction efficacy (RT-PCR). To test the transduction efficiency in vivo, Ad.BMP was injected into the quadriceps muscle of two animals. Quantitative real-time RT-PCR was performed comparing the animals from the BMP group 3 days after transduction (B1, B2) to untreated animals (N1, N2). To exclude systemic effects, the contralateral quadriceps of the treated animals was analyzed (K). Furthermore, we sacrificed one animal after muscle flap perfusion (L). Both flaps (B1, B2) showed increased BMP-2 levels compared to the contralateral quadriceps (K) or the flap of control animals (N1, N2).