Figure 1
Surgical site. The image demonstrates the elevated quadriceps muscle flap only attached by its pedicle with the Pravaz cannula in the afferent artery. The efferent vein is clamped and the microplate is displayed bridging the femoral critical-size defect. Via the Pravaz cannula, 1.0 ml of virus solution (8 × 109 plaque-forming units (BMP group) or 6 × 109 plaque-forming units (GFP group)) were injected (incubation time 1 h). A micro-plate (KLSMartin, Tuttlingen, Germany) secures the femur after performing a critical-size defect (5 mm) in the middle of the femoral shaft.