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Table 1 Procedure of PLA2 activity measurement

From: Effect of percutaneous nucleoplasty with coblation on phospholipase A2 activity in the intervertebral disks of an animal model of intervertebral disk degeneration: a randomized controlled trial

Regents

Tube A

Tube B

Substrate buffer solution

8 ml

8 ml

Calcium chloride (0.5 mol/L)

-

0.2 ml

EDTAa (15 mmol/L)

1.1 ml

-

Samples

0.4 ml

0.4 ml

 

Water bath at 37°C, 60 min

 

Calcium chloride (0.5 mol/L)

0.2 ml

-

EDTA (15 mmol/L)

-

1.1 ml

  1. One PLA2 activity unit was defined as follows: 1 ml of sample consumes 1 nmol of dilute hydrochloric acid solution per min during titration at 37°C. (1 U = l nmol ml/min). aEthylenediaminetetraacetic acid.
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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X