From: Effect of percutaneous nucleoplasty with coblation on phospholipase A2 activity in the intervertebral disks of an animal model of intervertebral disk degeneration: a randomized controlled trial
Regents
Tube A
Tube B
Substrate buffer solution
8 ml
Calcium chloride (0.5 mol/L)
-
0.2 ml
EDTAa (15 mmol/L)
1.1 ml
Samples
0.4 ml
Water bath at 37°C, 60 min
EDTA (15 mmol/L)