Table 1 List of PCR primers
Marker | Gene | Sequence (forward/reverse) | Ann. temp. (°C) | Fragment (bp) |
|---|---|---|---|---|
Premature | OCT-4 | 5′-GTCGCCAGAAGGGCAAAC-3′ | 57.0 | 157 |
5′-CAGGGTGGTGAAGTGAGGG-3′ | ||||
SOX-2 | 5′-CCCTGCAGTACAACTCCATGAC-3′ | 59.0 | 85 | |
5′-GGTGCCCTGCTGCGAGTA-3′ | ||||
KLF-4 | 5′-CGGCAAAACCTACACGAAGAGT-3′ | 59.0 | 119 | |
5′-AGTTCATCTGAGCGGGCAAAT-3′ | ||||
NANOG | 5′-CTTATTCAGGACAGCCCTGATTCTTC-3′ | 59.0 | 613 | |
5′-AAGACGGCCTCCAAATCACTG-3′ | ||||
C-MYC | 5′-GGATTCCGCCTCGTT-3′ | 55.1 | 184 | |
5′-TCTCCAAGCATCACTCG-3′ | ||||
Osteogenic | ALP | 5′-ATGAGCTCAACCGGAACAA-3′ | 56.0 | 131 |
5′-GTGCCCATGGTCAATCCT-3′ | ||||
OC | 5′-TCAACCCCGACTGCGACGAG-3′ | 68.0 | 204 | |
5′-TTGGAGCAGCTGGGATGATGG-3′ | ||||
ON | 5′-TCCGGATCTTTCCTTTGCTTTCTA-3′ | 57.5 | 187 | |
5′-CCTTCACATCGTGGCAAGAGTTTG-3′ | ||||
Chondrogenic | SOX-9 | 5′-CCGGTGCGCGTCAAC-3′ | 57.5 | 119 |
5′-TGCAGGTGCGGGTACTGAT-3′ | ||||
AGG | 5′-TTCCCTGAGGCCGAGAAC-3′ | 65.5 | 194 | |
5′-GGGCGGTAATGGAACACAAC-3′ | ||||
Adipogenic | PPAR-γ2 | 5′-GCGCCCTGGCAAAGCACT-3′ | 59.8 | 238 |
5′-TCCACGGAGCGAAACTGACAC-3′ | ||||
AP2 | 5′-GGCCAAACCCAACCTGA-3′ | 59.8 | 167 | |
5′-GGGCGCCTCCATCTAAG-3′ | ||||
Housekeeping | GAPDH | 5′-ACCACAGTCCATGCCATCAC-3′ | 60.0 | 450 |
5′-TCCACCACCCTGTTGCTGTA-3′ |