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Table 1 List of PCR primers

From: A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells

Marker Gene Sequence (forward/reverse) Ann. temp. (°C) Fragment (bp)
Premature OCT-4 5′-GTCGCCAGAAGGGCAAAC-3′ 57.0 157
5′-CAGGGTGGTGAAGTGAGGG-3′
SOX-2 5′-CCCTGCAGTACAACTCCATGAC-3′ 59.0 85
5′-GGTGCCCTGCTGCGAGTA-3′
KLF-4 5′-CGGCAAAACCTACACGAAGAGT-3′ 59.0 119
5′-AGTTCATCTGAGCGGGCAAAT-3′
NANOG 5′-CTTATTCAGGACAGCCCTGATTCTTC-3′ 59.0 613
5′-AAGACGGCCTCCAAATCACTG-3′
C-MYC 5′-GGATTCCGCCTCGTT-3′ 55.1 184
5′-TCTCCAAGCATCACTCG-3′
Osteogenic ALP 5′-ATGAGCTCAACCGGAACAA-3′ 56.0 131
5′-GTGCCCATGGTCAATCCT-3′
OC 5′-TCAACCCCGACTGCGACGAG-3′ 68.0 204
5′-TTGGAGCAGCTGGGATGATGG-3′
ON 5′-TCCGGATCTTTCCTTTGCTTTCTA-3′ 57.5 187
5′-CCTTCACATCGTGGCAAGAGTTTG-3′
Chondrogenic SOX-9 5′-CCGGTGCGCGTCAAC-3′ 57.5 119
5′-TGCAGGTGCGGGTACTGAT-3′
AGG 5′-TTCCCTGAGGCCGAGAAC-3′ 65.5 194
5′-GGGCGGTAATGGAACACAAC-3′
Adipogenic PPAR-γ2 5′-GCGCCCTGGCAAAGCACT-3′ 59.8 238
5′-TCCACGGAGCGAAACTGACAC-3′
AP2 5′-GGCCAAACCCAACCTGA-3′ 59.8 167
5′-GGGCGCCTCCATCTAAG-3′
Housekeeping GAPDH 5′-ACCACAGTCCATGCCATCAC-3′ 60.0 450
5′-TCCACCACCCTGTTGCTGTA-3′
  1. OCT-4 octamer-binding transcription factor 4, SOX-2 sex-determining region Y box 2; KLF-4 Krüppel-like factor 4, NANOG, homeobox protein NANOG, C-MYC, cellular myelocytomatosis oncogene, ALP alkaline phosphatase, OC osteocalcin, ON osteonectin, SOX-9 sex-determining region Y-box 9, AGG aggrecan, PPAR-γ2 peroxisome proliferator-activated receptor γ2, AP2 adipocyte fatty acid-binding protein 2, GAPDH glyceraldehyde-3-phosphate dehydrogenase.