Figure 5

Quantification of alizarin red staining during osteogenic cultures of HF-hMSCs on Ti discs and Ti screws. HF-hMSC populations were seeded onto Ti discs or Ti screws and cultured for up to 28 days in osteogenic medium. Matrix, which had been stained with alizarin red, was solubilised. Quantification results are presented as the absorbance at A₄₉₂ normalised to the amount of DNA per surface area of the Ti disc or Ti screw. Values represent means ± S.E.M; Data sets were not normally distributed and analysed by Kruskal-Wallis H test; N = 3 biological replicates where the average of each biological replicate was performed in technical replicates of three; asterisk denotes P < 0.05.