Figure 3

Analysis of DNA content and cell vitality of HF-hMSC populations during osteogenic culture on titanium discs and screws. HF-hMSC populations were seeded onto Ti discs and Ti screws and cultured for up to 28 days. (A) The amount of DNA within cultures was analysed using Picogreen® as a measure of cellular content, and results are presented as amount of DNA normalised to the surface area of the disc or screw. (B) Metabolic activity was analysed at day 28 using alamarBlue® as a measure of cellular vitality/health. Results are presented as resorufin fluorescence (Ex: 544/Em: 585) normalised to the amount of DNA per surface area of the disc or screw. Values represent means ± S.E.M; Data sets were not normally distributed and analysed by Kruskal-Wallis H test; N = 3 biological replicates where the average of each biological replicate was performed in technical replicates of three; asterisk denotes P < 0.05; N.S.: not significant.