Figure 7

Ihh differentially regulates the expression of markers of osteogenic, chondrogenic, angiogenic, and Ihh signaling genes. Consistent with the previous study, the expression of the earliest osteogenic marker gene, ALP, was almost always upregulated (a). Similarly, angiogenic factor (VEGF) and markers (CD31) were also mostly upregulated (c and d). Strangely, for unknown reasons, Col2a1 a chondrogenic marker was only marginally regulated by Ihh in C3H10T1/2 cells (b). Ptc1 and Smo, Ihh receptors, are upregulated by Ihh at most time points (e and f). In contrast, the expression of PTHrP, a factor in the negative feedback loop of Ihh signaling, was unchanged or suppressed at some time points in C3H10T1/2 cells (g). *p< 0.05, statistical significance (paired t-test, comparing with control group at the same time point).