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Figure 6 | Journal of Orthopaedic Surgery and Research

Figure 6

From: Mesenchymal stem cells overexpressing Ihh promote bone repair

Figure 6

Ihh promotes the expression of chondrogenic and osteogenic markers in vitro . Both C3H10T1/2 cells (a and b) and rabbit MSCs (c-f) were used in this study. Typical images demonstrated that under chondrogenic condition (a-d), strong Alcian Blue staining was observed in both EGFP+ Ihh+ C3H10T1/2 cells (b) and EGFP+ Ihh+ MSCs (d) 21 days after transfection, while only minimum Alcian Blue staining was observed in control groups (a and c). Similarly, under osteogenic condition (e and f), strong ALP+ staining was observed only in EGFP+ Ihh+ MSCs (f) not in MSCs group (e), 7 days after transfection. Scale bars = 100 μm.

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