Figure 1From: Mesenchymal stem cells overexpressing Ihh promote bone repairOverexpression of Ihh in C3H10T1/2 up-regulates Gli2 and Gli3 in the nuclei. Typical images of double staining demonstrated overexpression of Ihh (GFP+, green) co-localized with upregulated Gli2 and Gli3 (red), respectively. The first and third rows were none transfected cells, and the second and fourth rows were transfected cells. The first three columns are the split channels of DAPI (blue), GFP (green), Gli signals (red), respectively, and the fourth column is the merged images of the respective three split channels. Note that the most signals, both Gli2 and Gli3 and GFP, were in the nuclei (DAPI+, blue). Scale bars = 5 μm.Back to article page