Effects of initial boost with TGF-beta 1 and grade of intervertebral disc degeneration on 3D culture of human annulus fibrosus cells

Table 1 Oligonucleotide sequences

Gene name	Accession number	Oligonucleotides (5′ → 3′), (up/down)	Base pairs
Aggrecan	NM_001135	GGC TGC TGT CCC CGT AGA AGA/GGG AGG CCA AGT AGG AAG GAT	163
Biglycan	NM_001711	GCT GCC CCT GCT CTC CCA CCA CA/GAA ATG CAT GAG GAG GAG GAA CAG AAC	217
Collagen type I	NM_000088	CGA TGG CTG CAC GAG TCA CAC/CAG GTT GGG ATG GAG GGA GTT TAC	180
Collagen type II	NM_001844	CCG GGC AGA GGG CAA TAG CAG GTT/CAA TGA TGG GGA GGC GTG AG	128
Collagen type III	NM_000090	GGT TTT GCC CCG TAT TAT GGA/AGT TTC TAG CGG GGT TTT TAC GAG	142
Collagen type X	NM_000493	GAA CTC CCA GCA CGC AGA ATC C/GTG TTG GGT AGT GGG CCT TTT ATG	145
Decorin	NM_001920	ACT TCT GCC CAC CTG GAC ACA ACA C/AAT GGC AGA GCG CAC GTA GAC ACA	128
Glyceraldehyde-3-phospate dehydrogenase	NM_002046	GGC GAT GCT GGC GCT GAG TAC/TGG TCC ACA CCC ATG ACG A	149
Matrix metalloproteinase 2	NM_004530	TCC CTG CCC CTC CCT TCA AC/CCT TTC CAG CAG ACA CCA TCA CC	196
Matrix metalloproteinase 13	NM_002427	CAA AAA CGC CAG ACA AAT GTG ACC/GAT GCA GGC GCC AGA AGA ATC T	105

ISSN: 1749-799X