Figure 4

Gene expression analysis of slightly and severely degenerated AF cells cultured in 3D scaffolds. Gene expression data were stratified by degeneration status of AF cells and are given for slightly and severely degenerated AF cells cultured in monolayers and in PGA-PLGA-PVDF scaffolds (3D) for up to 21 days. Fold changes of genes coding extracellular matrix molecules and matrix modifying enzymes are given compared to their expression level found in monolayer expanded AF cells. Bars show the mean and standard deviation. Asterisks (*) indicate significant differential expression (p < 0.05) compared to monolayer cultures. Number sign (#) indicate significant differential expression (p < 0.05) compared to severely degenerated AF cells.