Table 1 Primers used for real-time RT-PCR
From: Effect of parathyroid hormone on early chondrogenic differentiation from mesenchymal stem cells
Gene | Direction | Sequences (5′–3′) |
|---|---|---|
Sox9 | Forward | TACGACTGGACGCTGGTGCC |
Sox9 | Reverse | CCGTTCTTCACCGACTTCCTCC |
Col2a1 | Forward | CTGACCTGACCTGATGATACC |
Col2a1 | Reverse | CACCAGATAGTTCCTGTCTCC |
Col10a1 | Forward | CGAGGTATGCTTGATCTG |
Col10a1 | Reverse | GACAGTCCAGTTCTTCAT |
Col1a1 | Forward | TGACTGGAAGAGCGGAGAGT |
Col1a1 | Reverse | TCTCTCCAAACCAGACGTGC |
PTH1R | Forward | CTCCTTCTCTGCTGCCCAGT |
PTH1R | Reverse | TGCTGTGTGCAGAACTTCCT |
GAPDH | Forward | TGAAGCAGGCATCTGAGGG |
GAPDH | Reverse | CGAAGGTGGAAGAGTGGGAG |