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Figure 1 | Journal of Orthopaedic Surgery and Research

Figure 1

From: Effect of parathyroid hormone on early chondrogenic differentiation from mesenchymal stem cells

Figure 1

Histological findings of chondrogenic pellet cultures of MSCs treated with PTH for 21 days. Upper panels (A–E) show Alcian Blue staining, and lower panels (F–J) show immunohistochemical staining of collagen type II. PTH was administrated at various doses: 0 (A, F), 0.1 (B, G), 1 (C, H), 10 (D, I), or 100 nM (E, J). Immunohistochemical staining of mouse cartilage tissue using anti-collagen type II antibody (K) and normal rabbit IgG (L, as a negative control). Note that no background staining is observed in the section incubated with normal rabbit IgG. Scale bars = 100 μm.

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