Identification of mesenchymal stem cells and osteogenic factors in bone marrow aspirate and peripheral blood for spinal fusion by flow cytometry and proteomic analysis

Table 2 2-DE and MALDI-TOF MS identification of proteins that are differentially expressed in BMA and PB

Spot	Protein name	Mr	pI	Score^a	Sequence coverage^b(%)	Trend
1	Hemoglobin subunit beta	16,102	6.75	68	57	BMA > PB
2	Catalase	59,947	6.9	206	53	BMA > PB
3	Ferritin light chain	20,064	5.51	142	63	BMA > PB
4	Flavin reductase	22,219	7.13	179	89	BMA > PB
5	Protein S100-A8	10,885	6.51	103	67	BMA > PB
6	Complement C3	188,569	6.02	66	11	BMA > PB
7	Fibrinogen beta chain	56,577	8.54	148	51	BMA > PB
8	Hemopexin	52,385	6.55	121	41	BMA < PB
9	Serotransferrin	79,280	6.81	92	25	BMA < PB
10	Transthyretin	15,991	5.52	155	73	BMA > PB
11	Clusterin	53,031	5.89	108	41	BMA > PB
12	Apolipoprotein A-I	30,759	5.56	113	49	BMA < PB
13	Peroxiredoxin-2	22,049	5.66	181	72	BMA > PB
14	Leucine-rich alpha-2-glycoprotein	38,382	6.45	89	42	BMA < PB
15	Fibrinogen gamma chain	52,106	5.37	131	48	BMA < PB
16	Glutathione peroxidase	25,765	8.26	67	39	BMA > PB

^aThe MASCOT search score of identified proteins is shown; ^b the percentage of sequence coverage (Seq Cov) of matched peptides in the identified protein.

ISSN: 1749-799X