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Figure 5 | Journal of Orthopaedic Surgery and Research

Figure 5

From: Identification of mesenchymal stem cells and osteogenic factors in bone marrow aspirate and peripheral blood for spinal fusion by flow cytometry and proteomic analysis

Figure 5

Protein profiling of BMA and PB specimens. (A) Proteins (150 mg) were subjected to 2-DE (pH 3 to 10) and detected by silver staining. Spots that had more than a 1.5-fold increase or decrease in silver staining were excised and in-gel digested with trypsin. At least 11 protein spots were detected at higher expression levels and 5 at lower expression levels in the BMA than in the PB. (B) Cropped images showing the two selected proteins (catalase and Gpx) from 2-D gels.

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