Figure 5From: Identification of mesenchymal stem cells and osteogenic factors in bone marrow aspirate and peripheral blood for spinal fusion by flow cytometry and proteomic analysisProtein profiling of BMA and PB specimens. (A) Proteins (150 mg) were subjected to 2-DE (pH 3 to 10) and detected by silver staining. Spots that had more than a 1.5-fold increase or decrease in silver staining were excised and in-gel digested with trypsin. At least 11 protein spots were detected at higher expression levels and 5 at lower expression levels in the BMA than in the PB. (B) Cropped images showing the two selected proteins (catalase and Gpx) from 2-D gels.Back to article page