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Table 3 Statistical analysis of gene expression profiles of 3D cartilage grafts in vitro

From: An ovine in vitro model for chondrocyte-based scaffold-assisted cartilage grafts

gene

point in time

rel. expression mean %GAPDH

SD

p-value vs. 1 week 3D

p-value vs. 2 weeks 3D

p-value vs. 3 weeks 3D

type 2α1 collagen

P3

0.34

0.15

0.0066

0.0039

0.0547

1 week 3D (P3)

1.50

1.00

---

0.0248

0.4258

2 weeks 3D (P3)

3.42

2.23

0.0248

---

0.9102

3 weeks 3D (P3)

5.09

6.78

0.4258

0.9102

---

P5

0.07

0.06

0.0104

0.0028

0.0039

1 week 3D (P5)

0.19

0.08

---

0.0288

0.0039

2 weeks 3D (P5)

0.40

0.21

0.0288

---

0.0160

3 weeks 3D (P5)

1.48

0.84

0.0039

0.0160

---

Aggrecan

P3

2.60

1.91

0.0153

0.0031

0.0547

1 week 3D (P3)

6.69

5.47

---

0.0094

0.4547

2 weeks 3D (P3)

16.91

12.05

0.0094

---

0.0039

3 weeks 3D (P3)

8.04

8.41

0.4547

0.0039

---

P5

1.05

0.32

0.0309

0.0039

0.0002

1 week 3D (P5)

2.16

1.11

---

0.0039

0.0039

2 weeks 3D (P5)

6.27

4.56

0.0039

---

0.9449

3 weeks 3D (P5)

6.41

2.29

0.0039

0.9449

---

MMP1

P3

0.34

0.12

0.0039

0.0305

0.0008

1 week 3D (P3)

3.96

4.48

---

0.0078

0.0039

2 weeks 3D (P3)

1.20

1.07

0.0078

---

0.0009

3 weeks 3D (P3)

0.13

0.16

0.0039

0.0009

---

P5

0.23

0.14

0.0039

0.0029

0.0002

1 week 3D (P5)

3.52

4.00

---

0.0039

0.0039

2 weeks 3D (P5)

0.57

0.30

0.0039

---

0.9449

3 weeks 3D (P5)

0.07

0.10

0.0039

0.9449

---

MMP2

P3

20.81

26.32

0.0030

0.0032

0.0218

1 week 3D (P3)

52.38

38.09

---

0.4360

0.0058

2 weeks 3D (P3)

57.71

37.25

0.4360

---

0.0159

3 weeks 3D (P3)

33.18

34.32

0.0058

0.0159

---

P5

14.42

10.10

0.0118

0.0232

0.0001

1 week 3D (P5)

28.02

20.84

---

0.4063

0.2593

2 weeks 3D (P5)

33.47

28.82

0.4063

---

0.8868

3 weeks 3D (P5)

34.49

12.90

0.2593

0.8868

---

MMP13

P3

1.65

1.42

0.0078

0.0276

0.0039

1 week 3D (P3)

23.86

18.88

---

0.0151

0.0039

2 weeks 3D (P3)

8.68

6.96

0.0151

---

0.2867

3 weeks 3D (P3)

4.78

4.75

0.0039

0.2867

---

P5

0.74

0.38

0.0008

0.0052

0.0557

1 week 3D (P5)

19.00

10.39

---

0.0011

0.0008

2 weeks 3D (P5)

1.87

1.08

0.0011

---

0.0020

3 weeks 3D (P5)

0.60

0.51

0.0008

0.0020

---

TIMP1

P3

497.9

211.8

0.3558

0.7333

0.0171

1 week 3D (P3)

561.2

189.7

---

0.5256

0.0015

2 weeks 3D (P3)

512.1

258.1

0.5256

---

0.0133

3 weeks 3D (P3)

303.6

240.8

0.0015

0.0133

---

P5

615.6

334.5

0.2188

0.5559

0.0391

1 week 3D (P5)

463.3

49.7

---

0.0364

0.3946

2 weeks 3D (P5)

676.0

229.4

0.0364

---

0.0023

3 weeks 3D (P5)

422.5

121.7

0.3946

0.0023

---

TIMP2

P3

83.0

46.5

0.2066

0.3109

0.2574

1 week 3D (P3)

119.4

43.7

---

0.6909

0.0848

2 weeks 3D (P3)

109.8

48.5

0.6909

---

0.0907

3 weeks 3D (P3)

63.4

53.1

0.0848

0.0907

---

P5

97.7

35.1

0.5837

0.4985

0.9227

1 week 3D (P5)

110.2

55.4

---

0.2950

0.4336

2 weeks 3D (P5)

86.1

19.3

0.2950

---

0.3490

 

3 weeks 3D (P5)

96.2

26.2

0.4336

0.3490

---

TIMP3

P3

83.2

30.5

0.0003

0.0008

0.0097

1 week 3D (P3)

204.0

67.5

---

0.0061

0.0078

2 weeks 3D (P3)

156.5

46.4

0.0061

---

0.0730

3 weeks 3D (P3)

99.8

98.9

0.0078

0.0730

---

P5

120.4

35.4

0.0488

0.0195

0.0097

1 week 3D (P5)

187.2

85.9

---

0.7876

0.1902

2 weeks 3D (P5)

197.7

69.9

0.7876

---

0.0888

3 weeks 3D (P5)

282.2

167.3

0.1902

0.0888

---

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X