An ovine in vitro model for chondrocyte-based scaffold-assisted cartilage grafts

Table 1 Oligonucleotides

gene name	reference sequence	oligonucleotides (5‘→3‘) (up/down)	product size (base pairs)
GAPDH	AF02943	TGG AGT CCA CTG GGG TCT TCA CTA/TTG CTG ACA ATC TTG AGG GTA TT	160
aggrecan	AF019758	TCG GGG TAG GTG GCG AGG AA/GGG CGG TTG GGG AGA CTT CAA	150
type ΙΙα1 collagen	AF138883	GCT GGT CAG AGG GGC ATC GTT/AGG GGG ACC TCG GTC TCC AGA T	129
MMP1	AF267156	CAT TCT ACT GAC ATT GGG GCT CTG/GAA TCG TAG TTA TGG CAT CAA AAG T	190
MMP2	NM_001166180	CTT CCA GGG CAC ATC TTA CGA CAG/ACA TGG GGC ACC TTC CGA GTT	160
MMP13	AF267157	TGC ATC CTC AGC AGG TTG AAG C/GTC ATG GGA AGG GTG CTC ATA GG	101
osteocalcin	NM_001040009	AGG GCA GCG AGG TGG TGA AG/GCC GTA GAA GCG CCG ATA GG	173
TIMP1	NM_001009319	ACA GGT CCC AGA ATC GCA GTG AG/CAG CAG CAT AGG TCT TGG TGA ATC C	147
TIMP2	NM_001166186	GGG CCA AAG CGG TCA GTA AGA AG/AGA GGA GGG GGC TGT GTA GAT AAA C	149
TIMP3	NM_001166187	CCA CAC GGA AGC CTC TGA AAG TC/AGG GTA GCC GAA ATT GGA GAG CA	280

ISSN: 1749-799X