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Table 1 Oligonucleotides

From: An ovine in vitro model for chondrocyte-based scaffold-assisted cartilage grafts

gene name reference sequence oligonucleotides (5‘→3‘) (up/down) product size (base pairs)
GAPDH AF02943 TGG AGT CCA CTG GGG TCT TCA CTA/TTG CTG ACA ATC TTG AGG GTA TT 160
aggrecan AF019758 TCG GGG TAG GTG GCG AGG AA/GGG CGG TTG GGG AGA CTT CAA 150
type ΙΙα1 collagen AF138883 GCT GGT CAG AGG GGC ATC GTT/AGG GGG ACC TCG GTC TCC AGA T 129
MMP1 AF267156 CAT TCT ACT GAC ATT GGG GCT CTG/GAA TCG TAG TTA TGG CAT CAA AAG T 190
MMP2 NM_001166180 CTT CCA GGG CAC ATC TTA CGA CAG/ACA TGG GGC ACC TTC CGA GTT 160
MMP13 AF267157 TGC ATC CTC AGC AGG TTG AAG C/GTC ATG GGA AGG GTG CTC ATA GG 101
osteocalcin NM_001040009 AGG GCA GCG AGG TGG TGA AG/GCC GTA GAA GCG CCG ATA GG 173
TIMP1 NM_001009319 ACA GGT CCC AGA ATC GCA GTG AG/CAG CAG CAT AGG TCT TGG TGA ATC C 147
TIMP2 NM_001166186 GGG CCA AAG CGG TCA GTA AGA AG/AGA GGA GGG GGC TGT GTA GAT AAA C 149
TIMP3 NM_001166187 CCA CAC GGA AGC CTC TGA AAG TC/AGG GTA GCC GAA ATT GGA GAG CA 280
  1. GAPDH glyceraldehyde-3-phosphate dehydrogenase.
  2. MMP matrix metalloproteinase.
  3. TIMP tissue inhibitor of metalloproteinases.