From: Is non-union of tibial shaft fractures due to nonculturable bacterial pathogens? A clinical investigation using PCR and culture techniques
Primer
Sequence
16S rRNA
Forward
5′-AGAGTTTGATCCTGGCTCAG-3′
Reverse
5′-CCCACTGCTGCCCGTAG-3′
GAPDH
5′-TCTGCCCCCTCTGCTGATGCCCCC-3′
5′-CCATCACGCCACAGTTTCCCGGAG-3′
Note. GAPDH
Glyceraldehydes-3-phosphate dehydrogenase