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Table 1 Nucleotide sequences of primers used to determine agents responsible for non-union following tibial shaft fractures

From: Is non-union of tibial shaft fractures due to nonculturable bacterial pathogens? A clinical investigation using PCR and culture techniques

Primer

Sequence

16S rRNA

 

Forward

5′-AGAGTTTGATCCTGGCTCAG-3′

Reverse

5′-CCCACTGCTGCCCGTAG-3′

GAPDH

 

Forward

5′-TCTGCCCCCTCTGCTGATGCCCCC-3′

Reverse

5′-CCATCACGCCACAGTTTCCCGGAG-3′

Note. GAPDH

Glyceraldehydes-3-phosphate dehydrogenase