Figure 2

Assessment of mesenchymal lineage differentiation potential of CSP. Von Kossa staining for documentation of matrix mineralization showed (A) that, at day 18, mineralization was evident in osteogenic induced cultures. (B) In non-induced cultures no matrix mineralization was detected. Oil Red O staining for documentation of adipogenic differentiation showed (C) that, at day 20, lipid vacuoles were evident in adipogenic-induced cultures. (D) In non-induced cultures no lipid vacuoles were detected. Type II collagen staining showed (E) that, at day 28, type II collagen was evident in chondrogenic induced CSP. (F) In non-induced cells no type II collagen was detected. These illustrations are representative for all donors.