The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization

Table 2 Relative abundance of gene expression in FGF-2 and BMP-2 treated cells.

	Non-treated		FGF-2 treated		BMP-2 treated		FGF-2 vs BMP-2
Gene	Average	SD	Average	SD	Average	SD	p-value
Collagen Type I	85,081.73	2,5316.39	**678.21	358.27	*170,243.43	24,493.77	0.0003
Fibronectin	55,827.93	1,2119.18	*28,432.19	1195.92	**239,750.67	23,464.19	0.0001
IGF1	3,249.41	689.70	**50.65	13.30	4,193.34	739.19	0.0006
RUNX2	349.09	40.63	**674.95	63.04	1,043.65	783.29	n.s.
VEGFA	109.49	38.86	**5,132.66	755.22	537.13	379.66	0.0007
TGFβ	93.08	10.55	**245.40	41.93	*185.20	38.34	n.s.
ALP	58.30	34.81	13.39	11.68	91.77	23.15	0.0064
OC	16.20	3.19	**1.38	0.65	*34.04	6.11	0.0008
Noggin	7.11	2.77	*1.61	0.49	2.41	1.76	n.s.
BMP-2	0.40	0.12	**0.06	0.01	0.38	0.05	0.0004
MMP3	0.03	0.03	**4.04	0.97	0.12	0.14	0.0023

This table shows the relative abundance of gene expression in mineralizing MC3T3-E1 cells after 24 hours of treatment with FGF-2 (5 ng) or BMP-2 (100 ng). Total RNA was harvested 24 hours after the addition using Qiagen RNeasy kit. A two-step RT-qPCR was preformed. Each data point represents the mean ± SD of three biological independent samples. *p < 0.05; **p < 0.01; ***p < 0.0001 against 0 hour control samples with 2 tail student t test.

ISSN: 1749-799X