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Table 2 Relative abundance of gene expression in FGF-2 and BMP-2 treated cells.

From: The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization

  Non-treated FGF-2 treated BMP-2 treated FGF-2 vs BMP-2
Gene Average SD Average SD Average SD p-value
Collagen Type I 85,081.73 2,5316.39 **678.21 358.27 *170,243.43 24,493.77 0.0003
Fibronectin 55,827.93 1,2119.18 *28,432.19 1195.92 **239,750.67 23,464.19 0.0001
IGF1 3,249.41 689.70 **50.65 13.30 4,193.34 739.19 0.0006
RUNX2 349.09 40.63 **674.95 63.04 1,043.65 783.29 n.s.
VEGFA 109.49 38.86 **5,132.66 755.22 537.13 379.66 0.0007
TGFβ 93.08 10.55 **245.40 41.93 *185.20 38.34 n.s.
ALP 58.30 34.81 13.39 11.68 91.77 23.15 0.0064
OC 16.20 3.19 **1.38 0.65 *34.04 6.11 0.0008
Noggin 7.11 2.77 *1.61 0.49 2.41 1.76 n.s.
BMP-2 0.40 0.12 **0.06 0.01 0.38 0.05 0.0004
MMP3 0.03 0.03 **4.04 0.97 0.12 0.14 0.0023
  1. This table shows the relative abundance of gene expression in mineralizing MC3T3-E1 cells after 24 hours of treatment with FGF-2 (5 ng) or BMP-2 (100 ng). Total RNA was harvested 24 hours after the addition using Qiagen RNeasy kit. A two-step RT-qPCR was preformed. Each data point represents the mean ± SD of three biological independent samples. *p < 0.05; **p < 0.01; ***p < 0.0001 against 0 hour control samples with 2 tail student t test.