Figure 3

Effect of GCT-CM on FAK phosphorylation in rat osteoclast precursor cells (rOPCs). rOPCs were harvested and cultured in a serum-free medium overnight. Then, the cells were washed and incubated for 5 min in the presence of various reagents (GCT-CM, VEGF and ZD4190). The samples were immunoblotted with anti-tyrosine phosphorylated FAK (anti-pY-FAK) or anti-FAK Abs.