Figure 1

RANKL and OPG mRNA levels in MG-63 cells after particle and alpha-CGRP treatment. Time course of (A) RANKL and (D) OPG mRNA expression of MG-63 cells after stimulation with different UHMWPE particle concentrations. Time course of UHMWPE particle-induced RANKL mRNA expression after treatment with different alpha-CGRP doses in (B) a cell-particle ratio of 1:100 and (C) a cell-particle ratio of 1:500. Time course of UHMWPE particle-induced OPG mRNA expression after treatment with different alpha-CGRP doses in (E) a cell-particle ratio of 1:100 and (F) a cell-particle ration of 1:500. Significant differences are marked. ((a) P < 0.05).