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Figure 1 | Journal of Orthopaedic Surgery and Research

Figure 1

From: A rapid method for the generation of uniform acellular bone explants: a technical note

Figure 1

Four representative micrographs from LDH labeled ovine cancellous bone sections visualized with Axioplan (Fluorescence 515-565 emission filter). A: Non-treatment control cultured for 2 days with presence of viable osteocytes; B: Positive treatment control incubated at 56°C over night prior to 2 days culture; C: Freeze-thawing treatment after 2 days culture showing no viable osteocytes; D: Representative image to show the method of analysis - bone matrix area is marked in red, viable osteocytes are marked in blue.

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