Figure 3

Characterization of endothelial cells. Panel (A) illustrated the VEGFR-2 expression on the induced ECs (200×). Over 95% of cells lighted up in dark-field microscope for cytoplasmic vWF following cultures in EC conditional medium (Panel B, 200×). After one-week incubation in a Matrigel™ basement membrane system, these cells proliferated and developed capillary-like 3-D structures (Panel C, 200×), suggesting functional endothelial phenotype.