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Figure 2 | Journal of Orthopaedic Surgery and Research

Figure 2

From: Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells

Figure 2

Characterizations of osteoblasts. Over 95% of induced osteoblasts expressed osteocalcin visualized by immunocytochemistry stains (Panel A, 200×). Alkaline phosphatase (ALP) activity of osteoblasts was assayed using an ALP kit and visualized as the pink color (Panel B, 200×). In addition, von Kossa staining was performed to reveal ossification nodules in the culture dishes of induced cells, as an indicator of osteoblasts function (Panel C, 200×).

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