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Table 1 Set of primers used for real-time quantitative PCR

From: In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA

Gene Primer sequences Ta* (°C) Amplicon size (bp)
MMP-3 (NM_002422) Forward: 5'-CTTTTGGCGAAAATCTCTCAG-3'
Reverse: 5'-AAAGAAACCCAAATGCTTCAA-3'
55 404
TIMP-3
(NM_000362)
Forward: 5'-AACTCCGACATCGTGATCCG-3'
Reverse: 5'-GTAGTAGCAGGACTTG ATCT-3'
59 347
COL2A1
(NM_001844)
Forward: 5'-CAACACTGCCAACGTCCAGAT-3'
Reverse: 5'-CTGCTTCGTCCAGATAGGCAAT-3'
59 106
AGG
(NM_013227)
Forward: 5'-ACTTCCGCTGGTCAGATGGA-3'
Reverse: 5'-TCTCGTGCCAGATCATCACC-3'
59 110
HAS-1
(NM_001523)
Forward 5'-CGGCCTGTTCCCCTTCTTCGTG-3'
Reward 5'-TCGTGTGCTACGCTGCGGACCA-3'
65 348
HAS-2
(NM_005328)
Forward 5'-CACAGCTGCTTATATTGTTG-3'
Reward 5'-AGTGGCTGATTTGTCTCTGC-3'
51 358
GAPDH
(NM_002046)
Forward: 5'-TGGTATCGTGGAAGGACTCAT-3'
Reverse: 5'-GTGGGTGTCGCTGTTGAAGTC-3'
58 370
  1. * Ta = Annealing temperature