Table 1 Set of primers used for real-time quantitative PCR
Gene | Primer sequences | Ta* (°C) | Amplicon size (bp) |
|---|---|---|---|
MMP-3 (NM_002422) | Forward: 5'-CTTTTGGCGAAAATCTCTCAG-3' Reverse: 5'-AAAGAAACCCAAATGCTTCAA-3' | 55 | 404 |
TIMP-3 (NM_000362) | Forward: 5'-AACTCCGACATCGTGATCCG-3' Reverse: 5'-GTAGTAGCAGGACTTG ATCT-3' | 59 | 347 |
COL2A1 (NM_001844) | Forward: 5'-CAACACTGCCAACGTCCAGAT-3' Reverse: 5'-CTGCTTCGTCCAGATAGGCAAT-3' | 59 | 106 |
AGG (NM_013227) | Forward: 5'-ACTTCCGCTGGTCAGATGGA-3' Reverse: 5'-TCTCGTGCCAGATCATCACC-3' | 59 | 110 |
HAS-1 (NM_001523) | Forward 5'-CGGCCTGTTCCCCTTCTTCGTG-3' Reward 5'-TCGTGTGCTACGCTGCGGACCA-3' | 65 | 348 |
HAS-2 (NM_005328) | Forward 5'-CACAGCTGCTTATATTGTTG-3' Reward 5'-AGTGGCTGATTTGTCTCTGC-3' | 51 | 358 |
GAPDH (NM_002046) | Forward: 5'-TGGTATCGTGGAAGGACTCAT-3' Reverse: 5'-GTGGGTGTCGCTGTTGAAGTC-3' | 58 | 370 |