Skip to main content
Figure 7 | Journal of Orthopaedic Surgery and Research

Figure 7

From: Vascular endothelial growth factor regulates osteoblast survival – evidence for an autocrine feedback mechanism

Figure 7

The effect of neutralisation of endogenous VEGF on primary human osteoblast BCl2 expression during spontaneous apoptosis in vitro. Expression of the antiapoptotic gene BCl2 was determined using Western immunoblotting as described in Materials and Methods. Spontaneous apoptosis was measured for cells in serum free conditions. BCl2: antiapoptotic gene, mAB VEGF: neutralising monoclonal antibody to vascular endothelial growth factor isotype 165 (0.3 ug/mL), control antibody: isotype control antibody with no biological activity in vitro. The immunoblot shown is representative in each case. Western Immunoblotting was performed for six separate experiments, with triplicates in each experiment.

Back to article page