Figure 6

The effect of exogenous VEGF 165 on primary human osteoblast BCl2 expression during spontaneous and 'pathological' apoptosis in vitro. Expression of the antiapoptotic gene BCl2 was determined using Western immunoblotting as described in Materials and Methods. Spontaneous apoptosis was measured for cells in serum free conditions. Pathological apoptosis was induced by treatment of the cultures with TNFa and anti-Fas IgM. BCl2: antiapoptotic gene, VEGF: vascular endothelial growth factor 165, TNFa: tumour necrosis factor alpha (100 ng/mL), anti-Fas IgM: anti Fas receptor immunoglobulin (1000 ng/mL). The immunoblot shown is representative in each case. Western Immunoblotting was performed for six separate experiments, with triplicates in each experiment.