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Figure 4 | Journal of Orthopaedic Surgery and Research

Figure 4

From: Multiple functions of the von Willebrand Factor A domain in matrilins: secretion, assembly, and proteolysis

Figure 4

Insertion of vWF A2 domain into MATN3 alters its oligomerization. A. Schematic diagram of Construct 1: MINI-MATN-3; and Construct 2: MINI-MATN-3 A2. B. Western blot analysis of the conditioned medium from Cos cells expressing (1) MINI-MATN-3, or (2) MINI-MATN-3, collected 72 hours after transfection. FLAG: analysis using the antiserum against the FLAG tag at the N-terminus of the recombinant matn-3. V5: analysis using the antiserum against the V5 tag at the C-terminus of the recombinant protein. Reducing conditions and the molecular weights of the Mini-Matn3 oligomers were indicated on the left, while the molecular weights of the Mini-Matn3 A2 oligomers are indicated on the right.

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