Sulphated-glycosaminoglycan (sGAG) production assay. The wet weight (Figure 6A) and sGAG production (Figure 6B) of the in vitro constructs were measured at 1, 2, and 3 weeks of culture, respectively. After 2 and 3 weeks in vitro, PLGA demonstrated significantly higher wet weight (p < 0.05) compared to fibrin/PLGA. The sGAG production in fibrin/PLGA construct was superior to PLGA. Relative sGAG contents (%) were significantly higher (p < 0.05) in fibrin/PLGA than PLGA at 1 week and 3 weeks.